Anti-Prolactin Receptor/PRLR Antibody Picoband®

About this product

Boster Bio Anti-Prolactin Receptor/PRLR Antibody Picoband® catalog # PB9782. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9782

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PRLR (Prolactin Receptor) is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al. (1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al. (1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues (histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR (conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.

Synonyms

Prolactin receptor; PRL-R; PRLR

Gene Name

PRLR

Gene ID

5618

UniProt

P16471

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PRLR, different from the related mouse sequence by eleven amino acids, and from the related rat sequence by fourteen amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in breast, placenta, kidney, liver and pancreas. .

Applications

WB

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors/Hormones, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This is a receptor for the anterior pituitary hormone prolactin (PRL) . Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. .

References & Citations

1. Arden, K. C., Boutin, J.-M., Djiane, J., Kelly, P. A., Cavenee, W. K. The receptors for prolactin and growth hormone are localized in the same region of human chromosome 5. Cytogenet. Cell Genet. 53: 161-165, 1990. 2. Arden, K. C., Cavenee, W. K., Boutin, J.-M., Kelly, P. A. The genes encoding the receptors for prolactin and growth hormone map to human chromosome 5. (Abstract) Am. J. Hum. Genet. 45 (suppl.) : A129 only, 1989. 3. Cunningham, B. C., Bass, S., Fuh, G., Wells, J. A. Zinc mediation of the binding of human growth hormone to the human prolactin receptor. Science 250: 1709-1712, 1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

69506 MW

Observed Molecular Weight

90 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Prolactin receptor

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Protein Name

Prolactin receptor

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.