Anti-RALB Antibody Picoband®

About this product

Boster Bio Anti-RALB Antibody Picoband® catalog # PB9795. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9795

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ras-related protein Ral-B (RalB) is a protein that in humans is encoded by the RALB gene on chromosome 2. This protein is one of two isoforms of the Ral protein, the other being RalA, and part of the Ras GTPase family. As a Ras GTPase, RalB functions as a molecular switch that becomes active when bound to GTP and inactive when bound to GDP. RalB can be activated by RalGEFs and, in turn, activate effectors in signal transduction pathways leading to biological outcomes. Additionally, Ral proteins have been associated with the progression of several cancers, including bladder cancer and prostate cancer. While the above functions appear to be shared between the two Ral isoforms, their differential subcellular localizations result in their differing involvement in certain biological processes. In particular, RalB is more involved in apoptosis and cell motility. Moreover, RalB specifically interacts with Exo84 to assemble the beclin-1–VPS34 autophagy initiation complex, and with Sec5 to activate the innate immune response via the Tank-binding kinase 1 (TBK1) .

Synonyms

Ras-related protein Ral-B; RALB

Gene Name

RALB

Gene ID

5899

UniProt

P11234

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human RALB, different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Cells of the B- and T-lymphocyte lineages.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Small G Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis. .

References & Citations

1. Entrez Gene: RALB v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein) . 2. Kashatus DF (Sep 2013) . Ral GTPases in tumorigenesis: emerging from the shadows. Experimental Cell Research 319 (15) : 2337–42. 3. Tecleab A, Zhang X, Sebti SM (Nov 2014) . Ral GTPase down-regulation stabilizes and reactivates p53 to inhibit malignant transformation. The Journal of Biological Chemistry 289 (45) : 31296–309.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23409 MW

Observed Molecular Weight

23 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Ras-related protein Ral-B

Subcellular Location

Cell membrane ; Lipid-anchor ; Cytoplasmic side . During late cytokinesis localizes at the midbody.

Protein Name

Ras-related protein Ral-B

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.