Anti-RASA1 Antibody Picoband®

About this product

Boster Bio Anti-RASA1 Antibody Picoband® catalog # PB9796. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9796

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

RASA1, also known as RAS p21 protein activator 1, is a 120-kDacytosolic human protein. The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Synonyms

Ras GTPase-activating protein 1; GAP; GTPase-activating protein; RasGAP; Ras p21 protein activator; p120GAP; RASA1; GAP, RASA

Gene Name

RASA1

Gene ID

5921

UniProt

P20936

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human RASA1, different from the related rat sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast) . Not detected in stromal, endothelial or Hofbauer cells (at protein level) . .

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Cancer, G Protein Signaling, Ras Family, Regulators, Signal Transduction, Signaling Pathway, Small G Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1. .

References & Citations

1. Entrez Gene: RASA1 RAS p21 protein activator (GTPase activating protein) 1. 2. Chow A, Gawler D (October 1999) . Mapping the site of interaction between annexin VI and the p120GAP C2 domain. FEBS Lett. 460 (1) : 166–72.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

116403 MW

Observed Molecular Weight

100 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Ras GTPase-activating protein 1

Subcellular Location

Cytoplasm .

Protein Name

Ras GTPase-activating protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.