Anti-GTPase HRAS Antibody Picoband®

About this product

Boster Bio Anti-GTPase HRAS Antibody catalog # RP1098. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1098

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

GTPase HRas, also known as transforming protein p21, is an enzyme that in humans is encoded by the HRAS gene. This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

Synonyms

GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; GTPase HRas, N-terminally processed; HRAS; HRAS1

Gene Name

HRAS

Gene ID

3265

UniProt

P01112

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GTPase HRAS, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

WB

Field of Research

Apoptosis, Associated Proteins, Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Differentiation, Epigenetics and Nuclear Signaling, G Protein Signaling, Intracellular, Nucleotide Messenger, Proto-Oncogenes, Ras Family, Second Messenger, Signal Transduction, Signaling Pathway, Small G Proteins, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. .

References & Citations

1. Entrez Gene: v-Ha-ras Harvey rat sarcoma viral oncogene homolog. 2. Russell MW, Munroe DJ, Bric E, Housman DE, Dietz-Band J, Riethman HC, Collins FS, Brody LC (July 1996) . A 500-kb physical map and contig from the Harvey ras-1 gene to the 11p telomere. Genomics 35 (2) : 353–60. 3. Wong-Staal F, Dalla-Favera R, Franchini G, Gelmann EP, Gallo RC (July 1981) . Three distinct genes in human DNA related to the transforming genes of mammalian sarcoma retroviruses. Science 213 (4504) : 226–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21298 MW

Observed Molecular Weight

21 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

GTPase HRas

Subcellular Location

Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity) . Shuttles between the plasma membrane and the Golgi apparatus. .

Protein Name

GTPase Hras

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.