Anti-Aquaporin 0/MIP Antibody Picoband®

About this product

Boster Bio Anti-Aquaporin 0/MIP Antibody Picoband® catalog # PB9811. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9811

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lens fiber major intrinsic protein, also called MIP26 or MP26, is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.

Synonyms

Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26; MIP; AQP0

Gene Name

MIP

Gene ID

4284

UniProt

P30301

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 0, different from the related mouse and rat sequences by six amino acids.

Clonality

Polyclonal

Tissue Specificity

Major component of lens fiber gap junctions.

Applications

WB

Field of Research

Immunology, Innate Immunity, Macrophage/Inflammation, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Water channel (PubMed:24120416) . Channel activity is down-regulated by CALM when cytoplasmic Ca (2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core (By similarity) . Plays a role in cell-to-cell adhesion and facilitates gap junction coupling (PubMed:24120416) . .

References & Citations

1. Berry, V., Francis, P., Kaushal, S., Moore, A., Bhattacharya, S. Missense mutations in MIP underlie autosomal dominant 'polymorphic' and lamellar cataracts linked to 12q. Nature Genet. 25: 15-17, 2000. 2. Francis, P., Chung, J.-J., Yasui, M., Berry, V., Moore, A., Wyatt, M. K., Wistow, G., Bhattacharya, S. S., Agre, P. Functional impairment of lens aquaporin in two families with dominantly inherited cataracts. Hum. Molec. Genet. 9: 2329-2334, 2000. 3. Pisano MM, Chepelinsky AB (Mar 1992) . Genomic cloning, complete nucleotide sequence, and structure of the human gene encoding the major intrinsic protein (MIP) of the lens. Genomics 11 (4) : 981–90.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28122 MW

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Lens fiber major intrinsic protein

Subcellular Location

Cell membrane ; Multi- pass membrane protein . Cell junction, gap junction .

Protein Name

Lens fiber major intrinsic protein

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.