Anti-RAP1A Antibody Picoband®

About this product

Boster Bio Anti-RAP1A Antibody Picoband® catalog # PB9816. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9816

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ras-related protein Rap-1A is a protein that in humans is encoded by the RAP1A gene. This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. And it is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs) . The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. Additionally, the encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.

Synonyms

Ras-related protein Rap-1A; C21KG; G-22K; GTP-binding protein smg p21A; Ras-related protein Krev-1; RAP1A; KREV1

Gene Name

RAP1A

Gene ID

5906

UniProt

P62834

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human RAP1A, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Overexpressed in bladder cancer cells (PubMed:17409436) . .

Applications

WB

Field of Research

Chromosome Structure, Epigenetics and Nuclear Signaling, G Protein Signaling, Nucleus, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF) -induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions. .

References & Citations

1. Entrez Gene: RAP1A RAP1A, member of RAS oncogene family. 2. Asha, H., de Ruiter, N. D., Wang, M.-G., Hariharan, I. K. The Rap1 GTPase functions as a regulator of morphogenesis in vivo.EMBO J. 18: 605-615, 1999. 3. Kawata M, Matsui Y, Kondo J, Hishida T, Teranishi Y, Takai Y (Dec 1988) . A novel small molecular weight GTP-binding protein with the same putative effector domain as the ras proteins in bovine brain membranes. Purification, determination of primary structure, and characterization. The Journal of Biological Chemistry263 (35) : 18965–71.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

20987 MW

Observed Molecular Weight

21 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Ras-related protein Rap-1A

Subcellular Location

Cell membrane ; Lipid-anchor . Cytoplasm . Cytoplasm, perinuclear region . Cell junction . Early endosome . Recruited from early endosome to late endosome compartment after nerve growth factor (NGF) stimulation. Localized with RAPGEF2 at cell-cell junctions (By similarity) . Colocalized with RAPGEF2 in the perinuclear region. .

Protein Name

Ras-related protein Rap-1A

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.