Anti-TIMP2 Antibody Picoband®

About this product

Boster Bio Anti-TIMP2 Antibody Picoband® catalog # PB9830. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9830

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

TIMP metallopeptidase inhibitor 2, a tissue inhibitor of metalloproteinases, also known as TIMP2, is a human gene, thought to be a metastasis suppressor. This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to ly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.

Synonyms

Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2; TIMP2

Gene Name

TIMP2

Gene ID

7077

UniProt

P16035

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human TIMP2 recombinant protein (Position: C27-P220) . Human TIMP2 shares 98.5% amino acid (aa) sequence identity with both mouse and rat TIMP2.

Clonality

Polyclonal

Tissue Specificity

Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.

Applications

WB

Field of Research

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. .

References & Citations

1. Entrez Gene: TIMP2 TIMP metallopeptidase inhibitor 2. 2. Bourboulia D, Stetler-Stevenson WG (Jun 2010) . Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) : Positive and negative regulators in tumor cell adhesion. Seminars in Cancer Biology 20 (3) : 161–8. 3. Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, Steingrimsson E (2008) . Novel MITF targets identified using a two-step DNA microarray strategy. Pigment Cell Melanoma Res. 21 (6) : 665–76.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

24399 MW

Observed Molecular Weight

24 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Metalloproteinase inhibitor 2

Subcellular Location

Secreted.

Protein Name

Metalloproteinase inhibitor 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.