Anti-Thymidine Kinase 1/TK1 Antibody Picoband®

About this product

Boster Bio Anti-Thymidine Kinase 1/TK1 Antibody Picoband® catalog # PB9832. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9832

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Thymidine kinase 1, soluble (gene name TK1), is a human thymidine kinase. Thymidine kinase catalyzes the phosphorylation of thymidine to deoxythymidine monophosphate. This gene is mapped to 17q23.2-q25.3. It is estimated that the maximal size of the TK1 gene is 14 kb and its minimal size between 4 and 5 kb. The gene contains many noncoding inserts and numerous Alu sequences. There are two forms of this protein, which have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Synonyms

Thymidine kinase, cytosolic;2.7.1.21; TK1

Gene Name

TK1

Gene ID

7083

UniProt

P04183

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human TK1 recombinant protein (Position: S2-N234) . Human TK1 shares 87.1% and 91.7% amino acid (aa) sequence identity with mouse and rat TK1, respectively.

Clonality

Polyclonal

Tissue Specificity

Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.

Applications

WB

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

References & Citations

1. Entrez Gene: Thymidine kinase 1, soluble. 2. Bradshaw, H. D., Jr., Deininger, P. L. Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells. Molec. Cell. Biol. 4: 2316-2320, 1984. 3. Kuo, W.-L., Hirschhorn, R., Huie, M. L., Hirschhorn, K. Localization and ordering of acid alpha-glucosidase (GAA) and thymidine kinase (TK1) by fluorescence in situ hybridization. Hum. Genet. 97: 404-406, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25469 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Thymidine kinase, cytosolic

Subcellular Location

Cytoplasm.

Protein Name

Thymidine kinase, cytosolic

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.