Anti-UPF3B/RENT3B Antibody Picoband®

About this product

Boster Bio Anti-UPF3B/RENT3B Antibody Picoband® catalog # PB9843. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9843

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Regulator of nonsense transcripts 3B is a protein that in humans is encoded by the UPF3B gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD) . When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.

Synonyms

Regulator of nonsense transcripts 3B; Nonsense mRNA reducing factor 3B; Up-frameshift suppressor 3 homolog B; hUpf3B; Up-frameshift suppressor 3 homolog on chromosome X; hUpf3p-X; UPF3B; RENT3B, UPF3X

Gene Name

UPF3B

Gene ID

65109

UniProt

Q9BZI7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human UPF3B /RENT3B.

Clonality

Polyclonal

Tissue Specificity

Expressed in testis, uterus, prostate, heart, muscle, brain, spinal cord and placenta. .

Applications

IHC, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, RNA Processing

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core. .

References & Citations

1. Entrez Gene: UPF3B UPF3 regulator of nonsense transcripts homolog B (yeast) . 2. Lykke-Andersen J, Shu MD, Steitz JA (Feb 2001) . Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon. Cell 103 (7) : 1121–31. 3. Serin G, Gersappe A, Black JD, Aronoff R, Maquat LE (Jan 2001) . Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4) . Mol Cell Biol 21 (1) : 209–23.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57762 MW

Observed Molecular Weight

58 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Regulator of nonsense transcripts 3B

Subcellular Location

Nucleus. Cytoplasm. Shuttling between the nucleus and the cytoplasm.

Protein Name

Regulator of nonsense transcripts 3B

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.