Anti-TRAP1 Antibody Picoband®

About this product

Boster Bio Anti-TRAP1 Antibody Picoband® catalog # PB9834. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9834

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heat shock protein 75 kDa, mitochondrial is a protein that in humans is encoded by the TRAP1 gene. It is mapped to 16p13.3. This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. And this protein may function in regulating cellular stress responses. In addition, it was found that TRAP1 interacted with the N-terminal half of TNFR1. Also, TRAP1 interacted with the C-terminal ends of the proteins encoded by both multiple exostoses-causing genes, EXT1 and EXT2, but not with EXTL1 or EXTL3.

Synonyms

Heat shock protein 75 kDa, mitochondrial; HSP 75; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1; TRAP1; HSP75

Gene Name

TRAP1

Gene ID

10131

UniProt

Q12931

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human TRAP1 recombinant protein (Position: A571-H704) . Human TRAP1 shares 91.7% and 94% amino acid (aa) sequence identity with mouse and rat TRAP1, respectively.

Clonality

Polyclonal

Tissue Specificity

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is higly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors. .

Applications

IHC, WB

Field of Research

Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. .

References & Citations

1. Entrez Gene: TRAP1 TNF receptor-associated protein 1. 2. Felts SJ, Owen BA, Nguyen P, Trepel J, Donner DB, Toft DO (March 2000) . The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties. J Biol Chem 275 (5) : 3305–12. 3. Song HY, Dunbar JD, Zhang YX, Guo D, Donner DB (March 1995) . Identification of a protein with homology to hsp90 that binds the type 1 tumor necrosis factor receptor. J Biol Chem 270 (8) : 3574–81.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

80110 MW

Observed Molecular Weight

80 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Heat shock protein 75 kDa, mitochondrial

Subcellular Location

Mitochondrion . Mitochondrion inner membrane . Mitochondrion matrix .

Protein Name

Heat shock protein 75 kDa, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.