Anti-TIF1 gamma/TRIM33 Antibody Picoband®

About this product

Boster Bio Anti-TIF1 gamma/TRIM33 Antibody Picoband® catalog # PB9836. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9836

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tripartite motif-containing 33 (TRIM33), also known as transcriptional intermediary factor 1 gamma (TIF1-γ), is a human gene. The TRIM33 gene is mapped to chromosome 1p13 by FISH. The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described; however, the full-length nature of one variant has not been determined.

Synonyms

E3 ubiquitin-protein ligase TRIM33;6.3.2.-; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33; TRIM33; KIAA1113, RFG7, TIF1G

Gene Name

TRIM33

Gene ID

51592

UniProt

Q9UPN9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human TIF1 gamma recombinant protein (Position: M1001-K1127) . Human TIF1 gamma shares 96.1% amino acid (aa) sequence identity with mouse TIF1 gamma.

Clonality

Polyclonal

Tissue Specificity

Expressed in stem cells at the bottom of the crypts of the colon (at protein level) . Expressed in colon adenomas and adenocarcinomas (at protein level) . Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Co-Factors, Epigenetics and Nuclear Signaling, Hematopoietic Progenitors, Nuclear, Signaling Pathways, Stem Cells, TGF Beta, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity) . Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade) . .

References & Citations

1. Entrez Gene: TRIM33 tripartite motif-containing 33. 2. He, W., Dorn, D. C., Erdjument-Bromage, H., Tempst, P., Moore, M. A. S., Massague, J. Hematopoiesis controlled by distinct TIF1-gamma and Smad4 branches of the TGF-beta pathway. Cell 125: 929-941, 2006. 3. Venturini, L., You, J., Stadler, M., Galien, R., Lallemand, V., Koken, M. H. M., Mattei, M. G., Ganser, A., Chambon, P., Losson, R., de The, H. TIF1-gamma, a novel member of the transcriptional intermediary factor 1 family. Oncogene 18: 1209-1217, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

122533 MW

Observed Molecular Weight

150 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

E3 ubiquitin-protein ligase TRIM33

Subcellular Location

Nucleus . In discrete nuclear dots resembling nuclear bodies. .

Protein Name

E3 ubiquitin-protein ligase TRIM33

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.