Anti-Apolipoprotein A I/APOA1 Antibody Picoband®

About this product

Boster Bio Anti-Apolipoprotein A I/APOA1 Antibody Picoband® catalog # PB9845. Tested in IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9845

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

Synonyms

Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1; Proapolipoprotein A-I; ProapoA-I; Apoa1

Gene Name

Apoa1

Gene ID

25081

UniProt

P04639

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived rat APOA1 recombinant protein (Position: D25-A259) . Rat APOA1 shares 61.7% and 68.6% amino acid (aa) sequence identity with human and mouse APOA1, respectively.

Clonality

Polyclonal

Tissue Specificity

Major protein of plasma HDL, also found in chylomicrons.

Applications

IHC, WB

Field of Research

Cardiovascular, Cholesterol Metabolism, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT) . As part of the SPAP complex, activates spermatozoa motility.

References & Citations

1. Brewer, H. B., Jr.; Fairwell, T.; LaRue, A.; Ronan, R.; Houser, A.; Bronzert, T. J. : The amino acid sequence of human apoA-I, an apolipoprotein isolated from high absorbance lipoproteins. Biochem. Biophys. Res. Commun. 80: 623-630, 1978. 2. Ma J, Liao XL, Lou B, Wu MP (2004) . Role of apolipoprotein A-I in protecting against endotoxin toxicity. Acta Biochim. Biophys. Sin. (Shanghai) 36 (6) : 419–24. 3. Yui Y, Aoyama T, Morishita H, Takahashi M, Takatsu Y, Kawai C (1988) . Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I (Apo A-I) . A novel function of Apo A-I. J. Clin. Invest. 82 (3) : 803–7. 4. Zhang, Y.; Zanotti, I.; Reilly, M. P.; Glick, J. M.; Rothblat, G. H.; Rader, D. J. : Overexpression of apolipoprotein A-I promotes reverse transport of cholesterol from macrophages to feces in vivo. Circulation 108: 661-663, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30062 MW

Observed Molecular Weight

24 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Apolipoprotein A-I

Subcellular Location

Secreted.

Protein Name

Apolipoprotein A-I

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.