Anti-BCAR3 Antibody Picoband®

About this product

Boster Bio Anti-BCAR3 Antibody Picoband® catalog # PB9846. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9846

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Breast cancer anti-estrogen resistance protein 3 is a protein that in humans is encoded by the BCAR3 gene. Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Breast cancer anti-estrogen resistance protein 3; Novel SH2-containing protein 2; SH2 domain-containing protein 3B; BCAR3; NSP2, SH2D3B; UNQ271/PRO308

Gene Name

BCAR3

Gene ID

8412

UniProt

O75815

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BCAR3, different from the related mouse sequence by five amino acids.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue. .

Applications

IHC, WB

Field of Research

Cell Type Markers, Tags & Cell Markers, Tumor Associated

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. .

References & Citations

1. Entrez Gene: BCAR3 breast cancer anti-estrogen resistance 3. 2. Dorssers LC, van Agthoven T, Brinkman A; et al. (2006) . Breast cancer oestrogen independence mediated by BCAR1 or BCAR3 genes is transmitted through mechanisms distinct from the oestrogen receptor signalling pathway or the epidermal growth factor receptor signalling pathway.. Breast Cancer Res. 7 (1) : R82–92. 3. van Agthoven T, van Agthoven TL, Dekker A, van der Spek PJ, Vreede L, Dorssers LC (Jul 1998) .Identification of BCAR3 by a random search for genes involved in antiestrogen resistance of human breast cancer cells. EMBO J 17 (10) : 2799–808.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

92566 MW

Observed Molecular Weight

93 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Breast cancer anti-estrogen resistance protein 3

Subcellular Location

Secreted.

Protein Name

Breast cancer anti-estrogen resistance protein 3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.