Anti-Cdc25C Antibody Picoband®

About this product

Boster Bio Anti-Cdc25C Antibody Picoband® catalog # PB9849. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9849

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

M-phase inducer phosphatase 3?is an?enzyme?that in humans is encoded by the?CDC25C?gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It s dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Synonyms

M-phase inducer phosphatase 3;3.1.3.48; Dual specificity phosphatase Cdc25C; CDC25C

Gene Name

CDC25C

Gene ID

995

UniProt

P30307

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc25C, different from the related mouse sequence by ten amino acids.

Clonality

Polyclonal

Tissue Specificity

Present only in testis (at protein level) . .

Applications

WB

Field of Research

Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. ly dephosphorylates CDK1 and activates its kinase activity. .

References & Citations

1. Entrez Gene: CDC25C cell division cycle 25 homolog C (S. pombe) . 2. Gould KL, Moreno S, Tonks NK, Nurse P (Feb 1991) . Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase. Science 250 (4987) : 1573–6. 3. Sartor H, Ehlert F, Grzeschik KH, et al. (1992) . Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively. Genomics 13 (3) : 911–2.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53365 MW

Observed Molecular Weight

53 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

M-phase inducer phosphatase 3

Subcellular Location

Nucleus .

Protein Name

M-phase inducer phosphatase 3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.