Anti-EGF Antibody Picoband®

About this product

Boster Bio Anti-EGF Antibody Picoband® catalog # PB9861. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9861

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms

Pro-epidermal growth factor; EGF; Epidermal growth factor; Urogastrone; EGF

Gene Name

EGF

Gene ID

1950

UniProt

P01133

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human EGF recombinant protein (Position: N971-R1023) . Human EGF shares 69.8% amino acid (aa) sequence identity with both mouse and rat EGF.

Clonality

Polyclonal

Tissue Specificity

Expressed in kidney, salivary gland, cerebrum and prostate. .

Applications

ELISA, WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941) . .

References & Citations

1. Brissenden, J. E., Ullrich, A., Francke, U.Chromosomal mapping of loci for insulin-like growth factors I and II and for epidermal growth factor in man.Am. J. Hum. Genet.36: 133S-only, 1984. 2. Carpenter G, Cohen S (May 1990) . Epidermal growth factor. The Journal of Biological Chemistry 265 (14) : 7709–12. 3. Herbst RS (2004) . Review of epidermal growth factor receptor biology. International Journal of Radiation Oncology, Biology, Physics 59 (2 Suppl) : 21–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

133994 MW

Observed Molecular Weight

134 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Pro-epidermal growth factor

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Pro-epidermal growth factor

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.