Anti-Fatty Acid Synthase/FASN Antibody Picoband®

About this product

Boster Bio Anti-Fatty Acid Synthase/FASN Antibody Picoband® catalog # PB9865. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9865

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fatty acid synthase  (FAS) is an  enzyme  that in humans is encoded by the  FASN  gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Synonyms

Fatty acid synthase;2.3.1.85; [Acyl-carrier-protein] S-acetyltransferase;2.3.1.38; [Acyl-carrier-protein] S-malonyltransferase;2.3.1.39;3-oxoacyl-[acyl-carrier-protein] synthase;2.3.1.41;3-oxoacyl-[acyl-carrier-protein] reductase;1.1.1.100;3-hydroxyacyl-[acyl-carrier-protein] dehydratase;4.2.1.59; Enoyl-[acyl-carrier-protein] reductase;1.3.1.39; Oleoyl-[acyl-carrier-protein] hydrolase;3.1.2.14; FASN; FAS

Gene Name

FASN

Gene ID

2194

UniProt

P49327

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human FASN recombinant protein (Position: M1-E226) . Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Prominent expression in brain, lung, and liver. .

Applications

IF, IHC, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Fatty Acid Oxidation, Fatty Acids, Integration Of Energy, Integration Of Energy Metabolism, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Neurology Process, Neuroscience, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

References & Citations

1. Entrez Gene: FASN fatty acid synthase. 2. Jayakumar A, Tai MH, Huang WY, al-Feel W, Hsu M, Abu-Elheiga L, Chirala SS, Wakil SJ (Oct 1995) . Human fatty acid synthase: properties and molecular cloning. Proc Natl Acad Sci U S A 92 (19) : 8695–9. 3. Smith S, Witkowski A, Joshi AK (July 2003) . Structural and functional organization of the animal fatty acid synthase. Prog. Lipid Res. 42 (4) : 289–317.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

273427 MW

Observed Molecular Weight

273 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Fatty acid synthase

Subcellular Location

Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Protein Name

Fatty acid synthase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.