Anti-Filaggrin/FLG Antibody Picoband®

About this product

Boster Bio Anti-Filaggrin/FLG Antibody Picoband® catalog # PB9870. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9870

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

In humans, profillagrin is encoded by the?FLG?gene, which is part of the S100 fused-type protein (SFTP) family within the?epidermal differentiation complex?on?chromosome 1q21. The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.

Synonyms

Filaggrin; FLG

Gene Name

FLG

Gene ID

2312

UniProt

P20930

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Filaggrin recombinant protein (Position: M1-R261) .

Clonality

Polyclonal

Tissue Specificity

Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417) . In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717) (PubMed:19384417) . .

Applications

IHC, WB

Field of Research

Cell Type Markers, Class I, Cytoskeleton, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Intermediate Filaments, Signal Transduction, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

References & Citations

1. Kypriotou, Magdalini; Huber, Marcel; Hohl, Daniel (September 2012) .The human epidermal differentiation complex: cornified envelope precursors, S100 proteins and the ‘fused genes’ family. Experimental Dermatology 21 (9) : 643–649. 2. Markova, NG; Marekov, LN; Chipev, CC; Gan, SQ; Idler, WW; Steinert, PM (January 1993) . Profilaggrin is a major epidermal calcium-binding protein.. Molecular and Cellular Biology 13 (1) : 613–25.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

435170 MW

Observed Molecular Weight

435 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Filaggrin

Subcellular Location

Cytoplasmic granule . In the stratum granulosum of the epidermis, localized within keratohyalin granules (PubMed:1429717) . In granular keratinocytes and in lower corneocytes, colocalizes with calpain-1/CAPN1 (PubMed:21531719) . .

Protein Name

Filaggrin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.