Anti-MDMX/MDM4 Antibody Picoband®

About this product

Boster Bio Anti-MDMX/MDM4 Antibody Picoband® catalog # PB9872. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9872

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein Mdm4?is a?protein?that in humans is encoded by the?MDM4?gene. This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.?

Synonyms

Protein Mdm4; Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4; MDM4; MDMX

Gene Name

MDM4

Gene ID

4194

UniProt

O15151

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MDMX, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues tested with high levels in thymus.

Applications

IHC, WB

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Death, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear, Nucleus, Pathways and Processes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. .

References & Citations

1. Entrez Gene: MDM4 Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse) . 2. Kadakia M, Brown TL, McGorry MM, Berberich SJ (Dec 2002) . MdmX inhibits Smad transactivation. Oncogene21 (57) : 8776–85. 3. Shvarts A, Bazuine M, Dekker P, Ramos YF, Steegenga WT, Merckx G, van Ham RC, van der Houven van Oordt W, van der Eb AJ, Jochemsen AG (Sep 1997) . Isolation and identification of the human homolog of a new p53-binding protein, Mdmx. Genomics 43 (1) : 34–42.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

54864 MW

Observed Molecular Weight

75 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Protein Mdm4

Subcellular Location

Nucleus.

Protein Name

Protein Mdm4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.