Anti-MGA Antibody Picoband®

About this product

Boster Bio Anti-MGA Antibody Picoband® catalog # PB9873. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9873

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mga  is a DNA-binding  protein  that activates the  expression  of several important  virulence  genes  in  Streptococcus pyogenes  (group A  Streptococcus, GAS) in response to changing environmental conditions. It had been found that the mouse transcription factor Max interacted with  Mga.  Coimmunoprecipitation analysis confirmed that Max and  Mga  interacted in transfected HEK293 cells. EMSA revealed that  Mga  required Max for binding to the E-box sequence CACGTG. The isolated T-box of  Mga  bound the brachyury T-box-binding site in the absence of Max.  Mga  repressed transcription of a reporter driven from a T-box-binding site, but coexpression of  Mga  with Max caused transcriptional activation from the T-box-binding site. Expression of  Mga  with Max, but not  Mga  alone, activated transcription from a reporter containing the E-box site.

Synonyms

MAX gene-associated protein; MAX dimerization protein 5; MGA; KIAA0518, MAD5

Gene Name

MGA

Gene ID

23269

UniProt

Q8IWI9

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MGA, different from the related mouse sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues tested with high levels in thymus.

Applications

WB

Field of Research

Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGGTGTGAAATT-3' core sequence and seems to regulate MYC-MAX target genes. Suppresses transcriptional activation by MYC and inhibits MYC-dependent cell transformation. Function activated by heterodimerization with MAX. This heterodimerization serves the dual function of both generating an E-box-binding heterodimer and simultaneously blocking interaction of a corepressor (By similarity) . .

References & Citations

1. Hurlin, P. J., Steingrimsson, E., Copeland, N. G., Jenkins, N. A., Eisenman, R. N. Mga, a dual-specificity transcription factor that interacts with Max and contains a T-domain DNA-binding motif. EMBO J. 18: 7019-7028, 1999. Note: Erratum: EMBO J. 19: 3841 only, 2000. 2. McIver KS, Myles RL (March 2002) . Two DNA-binding domains of Mga are required for virulence gene activation in the group A streptococcus. Mol. Microbiol. 43 (6) : 1591–601.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

331836 MW

Observed Molecular Weight

334 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

MAX gene-associated protein

Subcellular Location

Nucleus .

Protein Name

MAX gene-associated protein

Isotype

Rabbit IgG

Contents

243