Anti-Nestin Antibody Picoband®

About this product

Boster Bio Anti-Nestin Antibody Picoband® catalog # PB9874. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9874

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nestin  is a  type VI  intermediate filament  (IF) protein  that in humans is encoded by the NES  gene. This gene is mapped to 1q23.1. Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α -internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system (CNS) progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.

Synonyms

Nestin; NES; Nbla00170

Gene Name

NES

Gene ID

10763

UniProt

P48681

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Clonality

Polyclonal

Tissue Specificity

CNS stem cells.

Applications

IHC, WB

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cell Adhesion Proteins, Cell Type Marker, Developmental Biology, Intermediate Filaments, Intracellular, Invasion/Microenvironment, Lineage Markers, Lineage Specification, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Stem Cells, Tumor Antigens, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity) . .

References & Citations

1. Guérette D, Khan PA, Savard PE, Vincent M (2007) . Molecular evolution of type VI intermediate filament proteins. BMC Evol. Biol. 7: 164. 2. Michalczyk K, Ziman M (April 2005) . Nestin structure and predicted function in cellular cytoskeletal organisation. Histol. Histopathol. 20 (2) : 665–71.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

177439 MW

Observed Molecular Weight

230 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Nestin

Subcellular Location

Nucleus .

Protein Name

Nestin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.