Anti-Nucleobindin 2/NUCB2 Antibody Picoband®

About this product

Boster Bio Anti-Nucleobindin 2/NUCB2 Antibody Picoband® catalog # PB9876. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9876

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nucleobindin-2?is a?calcium-binding?EF-hand protein?that in humans is encoded by the?NUCB2?gene. By exon trapping and sequence analysis, ?the?NUCB2?sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.

Synonyms

Nucleobindin-2; DNA-binding protein NEFA; Prepronesfatin; Nesfatin-1; Nucb2; Nefa

Gene Name

Nucb2

Gene ID

53322

UniProt

P81117

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106) . Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.

Clonality

Polyclonal

Tissue Specificity

Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons. .

Applications

ELISA, IHC, WB

Field of Research

Calcium Binding Proteins, Calcium Signaling, Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calcium-binding protein. May have a role in calcium homeostasis. .

References & Citations

1. Entrez Gene: NUCB2 nucleobindin 2. 2. Barnikol-Watanabe S, Gross NA, Gotz H, Henkel T, Karabinos A, Kratzin H, Barnikol HU, Hilschmann N (February 1995) . Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein. Biol Chem Hoppe Seyler 375 (8) : 497–512. 3. DeAngelis, M., Drury, S., Doucet, J., Sherry, S., Buckley, L., Pelias, M., Deininger, P., Keats, B., Batzer, M. Construction of a BAC contig and isolation of candidate genes in the Acadian Usher syndrome (USH1C) critical region. (Abstract) Am. J. Hum. Genet. 61 (suppl.) : A233 only, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50304 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Nucleobindin-2

Subcellular Location

Cytoplasm . Perikaryon . Endoplasmic reticulum . Golgi apparatus . Nucleus envelope . Membrane ; Peripheral membrane protein . Secreted . In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted.

Protein Name

Nucleobindin-2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.