- Catalog number
- PB9877
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Serine/threonine-protein kinase PAK 3?is an?enzyme?that in humans is encoded by the?PAK3?gene. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47) . Alternatively spliced transcript variants encoding different isoforms have been identified.
- Synonyms
- Serine/threonine-protein kinase PAK 3;2.7.11.1; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3; PAK3; OPHN3
- Gene Name
- PAK3
- Gene ID
- 5063
- UniProt
- O75914
- Host
- Rabbit
- Reactivity
- Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human PAK3, identical to the related mouse and rat sequences.
- Clonality
- Polyclonal
- Tissue Specificity
- Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. .
- Applications
- WB
- Field of Research
- Apoptosis, Cancer, Cell Biology, G Protein Signaling, Host-Virus Interaction, Interspecies Interaction, Intracellular, Kinases, Microbiology, Neural Signal Transduction, Neurology Process, Neuroscience, Protein Phosphorylation, Ras Family, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction, Signaling Pathway, Small G Proteins
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. .
- References & Citations
- 1. Entrez Gene: PAK3 p21 (CDKN1A) -activated kinase 3. 2. Allen KM, Gleeson JG, Bagrodia S, Partington MW, MacMillan JC, Cerione RA, Mulley JC, Walsh CA (Oct 1998) . PAK3 mutation in nonsyndromic X-linked mental retardation. Nat Genet 20 (1) : 25–30. 3. Donnelly AJ, Partington MW, Ryan AK, Mulley JC (Nov 1996) . Regional localisation of two non-specific X-linked mental retardation genes (MRX30 and MRX31) . Am J Med Genet 64 (1) : 113–20.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 62 kDa
- Observed Molecular Weight
- 62 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Serine/threonine-protein kinase PAK 3
- Subcellular Location
- Cytoplasm .
- Protein Name
- Serine/threonine-protein kinase PAK 3
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
