Anti-DARPP32/PPP1R1B Antibody Picoband®

About this product

Boster Bio Anti-DARPP32/PPP1R1B Antibody Picoband® catalog # PB9879. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9879

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein phosphatase 1 regulatory subunit 1B (PPP1R1B), also known as dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP-32), is a protein that in humans is encoded by the PPP1R1B gene. This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B; DARPP32

Gene Name

PPP1R1B

Gene ID

84152

UniProt

Q9UD71

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human DARPP32, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. .

Applications

Flow Cytometry, IHC, WB

Field of Research

Adapters, Cytoplasmic, Metabolism, Neuroscience, Neurotransmission, Obesity, Receptors / Channels, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibitor of protein-phosphatase 1.

References & Citations

1. Entrez Gene: PPP1R1B protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32) . 2. Brené S, Lindefors N, Ehrlich M, Taubes T, Horiuchi A, Kopp J, Hall H, Sedvall G, Greengard P, Persson H (March 1994) . Expression of mRNAs encoding ARPP-16/19, ARPP-21, and DARPP-32 in human brain tissue. J. Neurosci. 14 (3 Pt 1) : 985–98. 3. Ouimet CC, Greengard P (February 1990) . Distribution of DARPP-32 in the basal ganglia: an electron microscopic study. J. Neurocytol. 19 (1) : 39–52.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

22963 MW

Observed Molecular Weight

32 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Protein phosphatase 1 regulatory subunit 1B

Subcellular Location

Cytoplasm.

Protein Name

Protein phosphatase 1 regulatory subunit 1B

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.