Anti-Prokineticin 1/PROK1 Antibody Picoband®

About this product

Boster Bio Anti-Prokineticin 1/PROK1 Antibody Picoband® catalog # PB9882. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9882

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Prokineticin-1 is also known as EG-VEGF. The International Radiation Hybrid Mapping Consortium mapped the PROK1 gene to chromosome 1. The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.

Synonyms

Prokineticin-1 ; Endocrine-gland-derived vascular endothelial growth factor ; EG-VEGF ; Prok1 ; Pk1

Gene Name

PROK1

Gene ID

246691

UniProt

Q14A28

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived mouse Prokineticin 1 recombinant protein (Position: A20-F105) . Mouse Prokineticin 1 shares 88.4% and 94.2% amino acid (aa) sequence identity with human and rat Prokineticin 1, respectively.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in liver and ovary and weakly expressed in testis and placenta. Expressed in mucosa and mesenchyme of embryonic gut during enteric nervous system development (at protein level) . Predominantly expressed in kidney and liver. Also expressed in lung, ovary, placenta and testis. In fetal liver, is restricted to and highly expressed in hepatocytes. In adult kidney, expression is restricted to the endothelial tubule cells. In placenta, expressed throughout gestation. .

Applications

WB

Field of Research

Growth Factors/Hormones, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. ly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. .

References & Citations

1. LeCouter, J., Kowalski, J., Foster, J., Hass, P., Zhang, Z., Dillard-Telm, L., Frantz, G., Rangell, L., DeGuzman, L., Keller, G.-A., Peale, F., Gurney, P., Hillan, K. J., Ferrara, N. Identification of an angiogenic mitogen selective for endocrine gland endothelium. Nature 412: 877-884, 2001. 2. Li, M., Bullock, C. M., Knauer, D. J., Ehlert, F. J., Zhou, Q. Y. Identification of two prokineticin cDNAs: recombinant proteins potently contract gastrointestinal smooth muscle. Molec. Pharm. 59: 692-698, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

11678 MW

Observed Molecular Weight

12 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Prokineticin-1

Subcellular Location

Secreted .

Protein Name

Prokineticin-1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.