Anti-Rab9/RAB9A Antibody Picoband®

About this product

Boster Bio Anti-Rab9/RAB9A Antibody Picoband® catalog # PB9884. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9884

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is ly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What’s more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.

Synonyms

Ras-related protein Rab-9A; RAB9A; RAB9

Gene Name

RAB9A

Gene ID

9367

UniProt

P51151

Host

Rabbit

Reactivity

Human, Monkey, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed; high expression found in skeletal muscle. .

Applications

Flow Cytometry, IP, IF, ICC, WB

Field of Research

G Protein Signaling, Organelles, Protein Trafficking, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins, Subcellular Markers, Tags & Cell Markers, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the transport of proteins between the endosomes and the trans Golgi network.

References & Citations

1. Carroll, K. S., Hanna, J., Simon, I., Krise, J., Barbero, P., Pfeffer, S. R. Role of Rab9 GTPase in facilitating receptor recruitment by TIP47. Science 292: 1373-1376, 2001. 2. Davies, J. P., Cotter, P. D., Ioannou, Y. A. Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics 41: 131-134, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

22838 MW

Observed Molecular Weight

23 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Ras-related protein Rab-9A

Subcellular Location

Cell membrane ; Lipid-anchor ; Cytoplasmic side . Endoplasmic reticulum membrane . Golgi apparatus membrane . Late endosome. Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasmic vesicle, phagosome. Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis.

Protein Name

Ras-related protein Rab-9A

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.