Anti-SIX1 Antibody Picoband®

About this product

Boster Bio Anti-SIX1 Antibody Picoband® catalog # PB9889. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9889

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Homeobox protein SIX1 (Sineoculis homeobox homolog 1) is a protein that in humans is encoded by the SIX1 gene. It is mapped to 14q23.1. The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3) .

Synonyms

Homeobox protein SIX1; Sine oculis homeobox homolog 1; SIX1

Gene Name

SIX1

Gene ID

6495

UniProt

Q15475

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SIX1, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Specifically expressed in skeletal muscle.

Applications

WB

Field of Research

Cancer Susceptibility, Cell Cycle, Cell Differentiation, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Proto-Oncogenes, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2 (By similarity) . Regulates the expression of CCNA1. .

References & Citations

1. Entrez Gene: SIX1 sine oculis homeobox homolog 1 (Drosophila) . 2. Boucher CA, Carey N, Edwards YH, Siciliano MJ, Johnson KJ (Apr 1996) . Cloning of the human SIX1 gene and its assignment to chromosome 14. Genomics 33 (1) : 140–2. 3. Ruf RG, Xu PX, Silvius D, Otto EA, Beekmann F, Muerb UT, Kumar S, Neuhaus TJ, Kemper MJ, Raymond RM, Brophy PD, Berkman J, Gattas M, Hyland V, Ruf EM, Schwartz C, Chang EH, Smith RJ, Stratakis CA, Weil D, Petit C, Hildebrandt F (May 2004) . SIX1 mutations cause branchio-oto-renal syndrome by disruption of EYA1-SIX1-DNA complexes. Proceedings of the National Academy of Sciences of the United States of America 101 (21) : 8090–5.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32210 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Homeobox protein SIX1

Subcellular Location

Nucleus . Cytoplasm.

Protein Name

Homeobox protein SIX1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.