Anti-SKAP55/SKAP1 Antibody Picoband®

About this product

Boster Bio Anti-SKAP55/SKAP1 Antibody Picoband® catalog # PB9890. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9890

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Src kinase-associated phosphoprotein 1 is an adapter protein that in humans is encoded by the SKAP1 gene. This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Synonyms

Src kinase-associated phosphoprotein 1; Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa; SKAP-55; pp55; SKAP1; SCAP1, SKAP55

Gene Name

SKAP1

Gene ID

8631

UniProt

Q86WV1

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SKAP55, different from the related mouse and rat sequences by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level) . .

Applications

IHC, WB

Field of Research

Adapters, Cell Type Markers, Immunoglobulins, Immunology, Non-CD, Protein Phosphorylation, Receptors, Signal Transduction, Transmembrane, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. .

References & Citations

1. Entrez Gene: SKAP1 src kinase associated phosphoprotein 1. 2. Marie-Cardine A, Bruyns E, Eckerskorn C, Kirchgessner H, Meuer SC, Schraven B (July 1997) . Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes. J Biol Chem 272 (26) : 16077–80. 3. Wang H, Moon EY, Azouz A, et al. (2003) . SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates. Nat. Immunol. 4 (4) : 366–74.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

41432 MW

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Src kinase-associated phosphoprotein 1

Subcellular Location

Cytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.

Protein Name

Src kinase-associated phosphoprotein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.