Anti-Staufen/STAU1 Antibody Picoband®

About this product

Boster Bio Anti-Staufen/STAU1 Antibody Picoband® catalog # PB9895. Tested in WB, ICC/IF applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9895

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Double-stranded RNA-binding protein Staufen homolog 1 is a protein that in humans is encoded by the STAU1 gene. Staufen is a member of the family of double-stranded RNA (dsRNA) -binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR.

Synonyms

Double-stranded RNA-binding protein Staufen homolog 1; STAU1; STAU

Gene Name

STAU1

Gene ID

6780

UniProt

O95793

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Staufen, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.

Applications

WB, ICC/IF

Field of Research

Cytoskeleton, Cytoskeleton/ECM, Microtubules, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Tubulin

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.

References & Citations

1. Entrez Gene: STAU1 staufen, RNA binding protein, homolog 1 (Drosophila) . 2. DesGroseillers L, Lemieux N (Feb 1997) . Localization of a human double-stranded RNA-binding protein gene (STAU) to band 20q13.1 by fluorescence in situ hybridization. Genomics 36 (3) : 527–9. 3. Kim YK, Furic L, Desgroseillers L, Maquat LE (Jan 2005) . Mammalian Staufen1 recruits Upf1 to specific mRNA 3'UTRs so as to elicit mRNA decay. Cell 120 (2) : 195–208.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

63182 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Double-stranded RNA-binding protein Staufen homolog 1

Subcellular Location

Cytoplasm . Rough endoplasmic reticulum . Localizes exclusively with the rough reticulum endoplasmic (RER) .

Protein Name

Double-stranded RNA-binding protein Staufen homolog 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.