Anti-YY1 Antibody Picoband®

About this product

Boster Bio Anti-YY1 Antibody Picoband® catalog # PB9909. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9909

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Synonyms

Transcriptional repressor protein YY1; Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1; YY-1; YY1; INO80S

Gene Name

YY1

Gene ID

7528

UniProt

P25490

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human YY1, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include activation or repression, in activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP) -mediated cardiac-specific gene expression (PubMed:15329343) . Binds to SMAD binding elements (SBEs) (5'- GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions) . .

References & Citations

1. Shi Y, Seto E, Chang LS, Shenk T (Oct 1991) . Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein. Cell 67 (2) : 377–88. 2. Thiaville MM, Kim J (2011) . Oncogenic potential of yin yang 1 mediated through control of imprinted genes. Critical Reviews in Oncogenesis 16 (3-4) : 199–209. 3. Zhu W, Lossie AC, Camper SA, Gumucio DL (Apr 1994) . Chromosomal localization of the transcription factor YY1 in the mouse and human. Mammalian Genome 5 (4) : 234–6.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

44713 MW

Observed Molecular Weight

65 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Transcriptional repressor protein YY1

Subcellular Location

Nucleus matrix . Associated with the nuclear matrix.

Protein Name

Transcriptional repressor protein YY1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.