Anti-ZP2 Antibody Picoband®

About this product

Boster Bio Anti-ZP2 Antibody Picoband® catalog # PB9912. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9912

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.

Synonyms

Zona pellucida sperm-binding protein 2; Zona pellucida glycoprotein 2; Zp-2; Zona pellucida protein A; Processed zona pellucida sperm-binding protein 2; ZP2; ZPA

Gene Name

ZP2

Gene ID

7783

UniProt

Q05996

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ZP2, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level) . In the spleen, mainly expressed in the white pulp germinal centers (at protein level) . Up-regulated in thymic lymphomas. .

Applications

IHC, WB

Field of Research

Developmental Biology, Reproduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. .

References & Citations

1. Entrez Gene: ZP2 zona pellucida glycoprotein 2 (sperm receptor) . 2. Burkart AD, Xiong B, Baibakov B, Jiménez-Movilla M, Dean J. Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.. J Cell Biol 197: 37–44. 3. Liang LF, Dean J (Apr 1993) . Conservation of mammalian secondary sperm receptor genes enables the promoter of the human gene to function in mouse oocytes. Dev Biol 156 (2) : 399–408.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

82357 MW

Observed Molecular Weight

82 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Zona pellucida sperm-binding protein 2

Subcellular Location

Processed zona pellucida sperm-binding protein 2: Secreted, extracellular space, extracellular matrix . The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida. .

Protein Name

Zona pellucida sperm-binding protein 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.