Anti-CD153/TNFSF8 Antibody

About this product

Boster Bio Anti-CD153/TNFSF8 Antibody catalog # RP1103. Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.

Specifications

Catalog number

RP1103

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD153 is a ligand for CD30. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Tumor necrosis factor ligand superfamily member 8; CD30 ligand; CD30-L; CD153; TNFSF8; CD30L, CD30LG

Gene Name

TNFSF8

Gene ID

944

UniProt

P32971

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CD153 recombinant protein (Position: Q63-D234) . Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

Flow Cytometry, IHC

Field of Research

Adaptive Immunity, Immunology, T Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

References & Citations

1. Cerutti, A., Schaffer, A., Goodwin, R. G., Shah, S., Zan, H., Ely, S., Casali, P. Engagement of CD153 (CD30 ligand) by CD30-positive T cells inhibits class switch DNA recombination and antibody production in human IgD-positive IgM-positive B cells. J. Immun. 165: 786-794, 2000. 2. Croager, E. J., Abraham, L. J. Characterisation of the human CD30 ligand gene structure. Biochim. Biophys. Acta 1353: 231-235, 1997. 3. Fischer, M., Harvima, I. T., Carvalho, R. F. S., Moller, C., Naukkarinen, A., Enblad, G., Nilsson, G. Mast cell CD30 ligand is upregulated in cutaneous inflammation and mediates degranulation-independent chemokine secretion. J. Clin. Invest. 116: 2748-2756, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26017 MW

Observed Molecular Weight

86 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Tumor necrosis factor ligand superfamily member 8

Subcellular Location

Membrane; Single-pass type II membrane protein.

Protein Name

Tumor necrosis factor ligand superfamily member 8

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.