Anti-ABL2 Antibody Picoband®

About this product

Boster Bio Anti-ABL2 Antibody Picoband® catalog # PB9913. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9913

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tyrosine-protein kinase ABL2, also known as Abelson-related gene (Arg), is an enzyme that in humans is encoded by the ABL2 gene. This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Synonyms

Abelson tyrosine-protein kinase 2;2.7.10.2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARG; ABL2; ABLL, ARG

Gene Name

ABL2

Gene ID

27

UniProt

P42684

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human ABL2 recombinant protein (Position: T1065-R1182) . Human ABL2 shares 97.5% amino acid (aa) sequence identity with mouse ABL2.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

IHC, WB

Field of Research

Cancer, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Signal Transduction, Transcription Factors, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Non-receptor tyrosine-protein kinase that plays an ABL1- overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement) ; CTTN (involved in signaling) ; or TUBA1 and TUBB (microtubule subunits) . Binds ly F-actin and regulates actin cytoskeletal structure through its F-actin- bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. .

References & Citations

1. Entrez Gene: ABL2 v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) . 2. Cao C, Leng Y, Li C, Kufe D (April 2003) . Functional interaction between the c-Abl and Arg protein-tyrosine kinases in the oxidative stress response. J. Biol. Chem. 278 (15) : 12961–7. 3. Kruh GD, King CR, Kraus MH, Popescu NC, Amsbaugh SC, McBride WO, Aaronson SA (Jan 1987) . A novel human gene closely related to the abl proto-oncogene. Science234 (4783) : 1545–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

128343 MW

Observed Molecular Weight

140 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Abelson tyrosine-protein kinase 2

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Name

Abelson tyrosine-protein kinase 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.