Anti-AFG3L2 Antibody Picoband®

About this product

Boster Bio Anti-AFG3L2 Antibody Picoband® catalog # PB9915. Tested in Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9915

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly. In humans, it is encoded by the AFG3L2 gene. This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. And this gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders as well as spastic ataxia-neuropathy syndrome.

Synonyms

AFG3-like protein 2;3.4.24.-; Paraplegin-like protein; AFG3L2

Gene Name

AFG3L2

Gene ID

10939

UniProt

Q9Y4W6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human AFG3L2 recombinant protein (Position: R168-D250) . Human AFG3L2 shares 100% amino acid (aa) sequence identity with mouse AFG3L2.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Highly expressed in the cerebellar Purkinje cells. .

Applications

Flow Cytometry, IP, IF, ICC, WB

Field of Research

Cell Biology, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

ATP-dependent protease which is essential for axonal development. .

References & Citations

1. Entrez Gene: AFG3 ATPase family gene 3-like 2 (S. cerevisiae) . 2. Koppen, M., Metodiev, M. D., Casari, G., Rugarli, E. I., Langer, T. Variable and tissue-specific subunit composition of mitochondrial m-AAA protease complexes linked to hereditary spastic paraplegia. Molec. Cell. Biol. 27: 758-767, 2007. 3. Pierson TM, Adams D, Bonn F, Martinelli P, Cherukuri PF, Teer JK, Hansen NF, Cruz P, Mullikin For The Nisc Comparative Sequencing Program JC, Blakesley RW, Golas G, Kwan J, Sandler A, Fuentes Fajardo K, Markello T, Tifft C, Blackstone C, Rugarli EI, Langer T, Gahl WA, Toro C (October 2011) . Whole-exome sequencing identifies homozygous AFG3L2 mutations in a spastic ataxia-neuropathy syndrome linked to mitochondrial m-AAA proteases. PLoS Genet. 7 (10) : e1002325.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

88584 MW

Observed Molecular Weight

89 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

AFG3-like protein 2

Subcellular Location

Mitochondrion membrane ; Multi-pass membrane protein .

Protein Name

AFG3-like protein 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.