Anti-Osteocalcin/BGLAP Antibody

About this product

Boster Bio Anti-Osteocalcin/BGLAP Antibody Picoband® catalog # PB9918. Tested in IHC applications. This antibody reacts with Human.

Specifications

Catalog number

PB9918

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time ing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.

Synonyms

Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; BGLAP

Gene Name

BGLAP

Gene ID

632

UniProt

P02818

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Osteocalcin recombinant protein (Position: Y52-V100) . Human Osteocalcin shares 69% and 80.9% amino acid (aa) sequence identity with mouse and rat Osteocalcin, respectively.

Clonality

Polyclonal

Tissue Specificity

Bcl-X (S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X (L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Applications

IHC

Field of Research

Cytoskeleton/ECM, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

References & Citations

1. Cancela L, Hsieh CL, Francke U, Price PA (September 1990) . Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene. J. Biol. Chem. 265 (25) : 15040–8. 2. Lee NK, Sowa H, Hinoi E, Ferron M, Ahn JD, Confavreux C, Dacquin R, Mee PJ, McKee MD, Jung DY, Zhang Z, Kim JK, Mauvais-Jarvis F, Ducy P, Karsenty G (August 2007) . Endocrine regulation of energy metabolism by the skeleton. Cell 130 (3) : 456–69. 3. Puchacz E, Lian JB, Stein GS, Wozney J, Huebner K, Croce C (May 1989) . Chromosomal localization of the human osteocalcin gene. Endocrinology 124 (5) : 2648–50.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10963 MW

Observed Molecular Weight

29 kDa, 60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Osteocalcin

Subcellular Location

Secreted.

Protein Name

Osteocalcin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.