Anti-CCT3 Antibody Picoband®

About this product

Boster Bio Anti-CCT3 Antibody Picoband® catalog # PB9926. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9926

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

T-complex protein 1 subunit gamma is a protein that in humans is encoded by the CCT3 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC) . This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8.

Synonyms

T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCT3; CCTG, TRIC5

Gene Name

CCT3

Gene ID

7203

UniProt

P49368

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CCT3, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Chaperones, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. .

References & Citations

1. Entrez Gene: CCT3 chaperonin containing TCP1, subunit 3 (gamma) . 2. Joly EC, Sévigny G, Todorov IT, Bibor-Hardy V (Mar 1994) . cDNA encoding a novel TCP1-related protein.Biochimica et Biophysica Acta 1217 (2) : 224–6. 3. Walkley NA, Demaine AG, Malik AN (Jan 1996) .Cloning, structure and mRNA expression of human Cctg, which encodes the chaperonin subunit CCT gamma. The Biochemical Journal 313 (Pt 2) : 381–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60534 MW

Observed Molecular Weight

61 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

T-complex protein 1 subunit gamma

Subcellular Location

Cytoplasm.

Protein Name

T-complex protein 1 subunit gamma

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.