Anti-TCP1 epsilon/CCT5 Antibody Picoband®

About this product

Boster Bio Anti-TCP1 epsilon/CCT5 Antibody Picoband® catalog # PB9928. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9928

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CCT5 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC) . This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP) . Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13.

Synonyms

T-complex protein 1 subunit epsilon; TCP-1-epsilon; CCT-epsilon; CCT5; CCTE, KIAA0098

Gene Name

CCT5

Gene ID

22948

UniProt

P48643

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human CCT5 recombinant protein (Position: L443-K535) . Human CCT5 shares 95.7% amino acid (aa) sequence identity with both mouse and rat CCT5.

Clonality

Polyclonal

Tissue Specificity

Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Applications

Flow Cytometry, IP, IF, IHC, ICC, WB

Field of Research

Actin Assembly, Actin, etc., Chaperones, Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Microtubules, Protein Trafficking, Signal Transduction, Tubulin

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. .

References & Citations

1. Bouhouche, A., Benomar, A., Bouslam, N., Chkili, T., Yahyaoui, M. Mutation in the epsilon subunit of the cytosolic chaperonin-containing t-complex peptide-1 (Cct5) gene causes autosomal recessive mutilating sensory neuropathy with spastic paraplegia. J. Med. Genet. 43: 441-443, 2006. 2. Liou, A. K. F., Willison, K. R. Elucidation of the subunit orientation in CCT (chaperonin containing TCP1) from the subunit composition of CCT micro-complexes. EMBO J. 16: 4311-4316, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

59671 MW

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

T-complex protein 1 subunit epsilon

Subcellular Location

Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .

Protein Name

T-complex protein 1 subunit epsilon

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.