Anti-CD2/CD2 Antibody Picoband®

About this product

Boster Bio Anti-CD2/CD2 Antibody Picoband® catalog # PB9929. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9929

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies ed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portion. The localization of CD2 to 1p13 is established by in situ hybridization. CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR is identified within the 3-prime flanking region of the human CD2 gene.

Synonyms

T-cell surface antigen CD2; LFA-2; LFA-3 receptor; Lymphocyte antigen 37; Ly-37; T-cell surface antigen T11/Leu-5; CD2; Cd2; Ly-37

Gene Name

Cd2

Gene ID

914

UniProt

P08920

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived mouse CD2 recombinant protein (Position: R23-S203) . Mouse CD2 shares 49.7% and 73.3% amino acid (aa) sequence identity with human and rat CD2, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Applications

IHC, WB

Field of Research

Stem Cells, T Cells, T Lymphocytic Lineage

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

References & Citations

1. Brown, M. H., Gorman, P. A., Sewell, W. A., Spurr, N. K., Sheer, D., Crumpton, M. J. The gene coding for the human T-lymphocyte CD2 antigen is located on chromosome 1p. Hum. Genet. 76: 191-195, 1987. 2. Clayton, L. K., Ramachandran, H., Pravtcheva, D., Chen, Y.-F., Diamond, D. J., Ruddle, F. H., Reinherz, E. L. The gene for T11 (CD2) maps to chromosome 1 in humans and to chromosome 3 in mice. J. Immun. 140: 3617-3621, 1988. 3. Kingsmore, S. F., Watson, M. L., Moseley, W. S., Seldin, M. F. Physical linkage of genes encoding the lymphocyte adhesion molecules CD2 and its ligand LFA-3. Immunogenetics 30: 123-125, 1989.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38415 MW

Observed Molecular Weight

58 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

T-cell surface antigen CD2

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

T-cell surface antigen CD2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.