Anti-CD9 Antibody Picoband®

About this product

Boster Bio Anti-CD9 Antibody Picoband® catalog # PB9930. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9930

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization. While oocytes are ovulated, CD9-deficient oocytes are not properly fused with sperm upon fertilization. CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.

Synonyms

CD9 antigen; CD9; Cd9

Gene Name

Cd9

Gene ID

12527

UniProt

P40240

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived mouse CD9 recombinant protein (Position: T110-I193) . Mouse CD9 shares 77.4% and 86.9% amino acid (aa) sequence identity with human and rat CD9, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed predominantly in the peripheral nervous system. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cardiovascular, Cell Type Markers, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Immunology, Intracellular, Platelets, Reproduction, Stem Cells, Surface Molecules

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity) . Acts as a receptor for PSG17. .

References & Citations

1. Katz F, Povey S, Parkar M, Schneider C, Sutherland R, Stanley K, Solomon E, Greaves M (March 1984) . Chromosome assignment of monoclonal antibody-defined determinants on human leukemic cells. Eur J Immunol 13 (12) : 1008–1013. 2. Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C (2000) . Severely Reduced Female Fertility in CD9-Deficient Mice. Science 287 (5451) : 319–321. 3. Runge KE, Evans JE, He ZY, Gupta S, McDonald KL, Stahlberg H, Primakoff P, Myles DG (2007) . Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution.Developmental Biology 304 (1) : 317–325.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25258 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

CD9 antigen

Subcellular Location

Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Secreted, exosome .

Protein Name

CD9 antigen

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.