Anti-CDCP1 Antibody Picoband®

About this product

Boster Bio Anti-CDCP1 Antibody Picoband® catalog # PB9933. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9933

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases) . CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.

Synonyms

CUB domain-containing protein 1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein; SIMA135; Transmembrane and associated with src kinases; CD318; CDCP1; TRASK; UNQ2486/PRO5773

Gene Name

CDCP1

Gene ID

64866

UniProt

Q9H5V8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human CDCP1 recombinant protein (Position: R582-T667) . Human CDCP1 shares 84.5% amino acid (aa) sequence identity with mouse CDCP1.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. .

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cell Type Markers, Extracellular Matrix, Hematopoietic Progenitors, Invasion/Microenvironment, Stem Cells, Surface Molecules, Tags & Cell Markers, Tumor Associated, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. .

References & Citations

1. Entrez Gene: CDCP1 CUB domain containing protein 1. 2. Scherl-Mostageer M, Sommergruber W, Abseher R, Hauptmann R, Ambros P, Schweifer N (Jul 2001) . Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer. Oncogene 20 (32) : 4402–8. 3. Spassov DS, Ahuja D, Wong CH, Moasser MM (2011) . The structural features of trask that mediate its anti-adhesive functions. PLoS ONE 6 (4) : e19154.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

92932 MW

Observed Molecular Weight

130 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

CUB domain-containing protein 1

Subcellular Location

Isoform 1: Cell membrane ; Single-pass membrane protein . Shedding may also lead to a soluble peptide.

Protein Name

CUB domain-containing protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.