Anti-Factor I/CFI Antibody Picoband®

About this product

Boster Bio Anti-Factor I/CFI Antibody Picoband® catalog # PB9935. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9935

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Complement factor I, also known as C3b/C4b inactivator, is a protein that in humans is encoded by the CFI gene. This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene.

Synonyms

Complement factor I;3.4.21.45; C3B/C4B inactivator; Complement factor I heavy chain; Complement factor I light chain; CFI; IF

Gene Name

CFI

Gene ID

3426

UniProt

P05156

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Factor I recombinant protein (Position: K19-D220) . Human Factor I shares 70.7% and 71.2% amino acid (aa) sequence identity with mouse and rat Factor I, respectively.

Clonality

Polyclonal

Tissue Specificity

Plasma.

Applications

Flow Cytometry, IHC, ICC, WB

Field of Research

Complement, Immunology, Innate Immunity

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.

References & Citations

1. Entrez Gene: complement factor I. 2. Goldberger G, Bruns GA, Rits M, Edge MD, Kwiatkowski DJ (Jul 1987) . Human complement factor I: analysis of cDNA-derived primary structure and assignment of its gene to chromosome 4. The Journal of Biological Chemistry 262 (21) : 10065–71.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

65750 MW

Observed Molecular Weight

75 kDa, 45 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Complement factor I

Subcellular Location

Secreted, extracellular space.

Protein Name

Complement factor I

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.