Anti-Alpha A Crystallin/CRYAA Antibody Picoband®

About this product

Boster Bio Anti-Alpha A Crystallin/CRYAA Antibody Picoband® catalog # PB9940. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9940

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC) .

Synonyms

Alpha-crystallin A chain; Heat shock protein beta-4; HspB4; Alpha-crystallin A (1-172) ; Alpha-crystallin A (1-168) ; Alpha-crystallin A (1-162) ; CRYAA; CRYA1, HSPB4

Gene Name

CRYAA

Gene ID

1409

UniProt

P02489

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173) . Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse and rat Alpha A Crystallin.

Clonality

Polyclonal

Tissue Specificity

Expressed in eye lens. .

Applications

Flow Cytometry, IHC, IHC-F, ICC, WB

Field of Research

Cell Type Markers, Chaperones, Heat Shock Proteins, Neuroscience, Protein Trafficking, Sensory System, Signal Transduction, Tags & Cell Markers, Visual System

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. .

References & Citations

1. Entrez Gene: CRYAA crystallin, alpha A. 2. Derham BK, Harding JJ (1999) . Alpha-crystallin as a molecular chaperone.. Progress in retinal and eye research 18 (4) : 463–509. 3. Jaworski CJ, Piatigorsky J (1989) . A pseudo-exon in the functional human alpha A-crystallin gene.. Nature337 (6209) : 752–4.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

19909 MW

Observed Molecular Weight

20 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Alpha-crystallin A chain

Subcellular Location

Cytoplasm . Nucleus . Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Protein Name

Alpha-crystallin A chain

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.