Anti-GADD45A Antibody Picoband®

About this product

Boster Bio Anti-GADD45A Antibody Picoband® catalog # PB9945. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9945

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Growth arrest and DNA-damage-inducible protein GADD45 alpha (GADD45A) is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. It is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. In addition, Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.

Synonyms

Growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DDIT-1; GADD45A; DDIT1, GADD45

Gene Name

GADD45A

Gene ID

1647

UniProt

P24522

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GADD45A, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. .

Applications

WB

Field of Research

DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity) . Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. .

References & Citations

1. Barreto, G., Schafer, A. Marhold, J., Stach, D., Swaminathan, S. K., Handa, V., Doderlein, G., Maltry, N., Wu, W., Lyko, F., Niehrs, C.Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.Nature445: 671-675, 2007. 2. Papathanasiou MA, Kerr NC, Robbins JH, McBride OW, Alamo I Jr, Barrett SF, Hickson ID, Fornace AJ Jr (Mar 1991) . Induction by ionizing radiation of the gadd45 gene in cultured human cells: lack of mediation by protein kinase C. Mol Cell Biol 11 (2) : 1009–16.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

18336 MW

Observed Molecular Weight

19 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Growth arrest and DNA damage-inducible protein GADD45 alpha

Subcellular Location

Nucleus .

Protein Name

Growth arrest and DNA damage-inducible protein GADD45 alpha

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.