Anti-Integrin linked ILK Antibody Picoband®

About this product

Boster Bio Anti-Integrin linked ILK Antibody Picoband® catalog # PB9949. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9949

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.

Synonyms

Integrin-linked protein kinase;2.7.11.1;59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK; ILK; ILK1, ILK2

Gene Name

ILK

Gene ID

3611

UniProt

Q13418

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Integrin linked ILK recombinant protein (Position: M1-H203) . Human Integrin linked ILK shares 99.5% amino acid (aa) sequence identity with both mouse and rat Integrin linked ILK.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

Applications

WB

Field of Research

Protein Phosphorylation, Signal Transduction, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

References & Citations

1. Entrez Gene: ILK integrin-linked kinase. 2. Fielding AB, Dobreva I, McDonald PC, Foster LJ, Dedhar S (Feb 2008) . Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. The Journal of Cell Biology 180 (4) : 681–9. 3. Melchior, C., Kreis, S., Janji, B., Kieffer, N. Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1. Biochim. Biophys. Acta 1575: 117-122, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51419 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Integrin-linked protein kinase

Subcellular Location

Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere.

Protein Name

Integrin-linked protein kinase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.