Anti-MEF2A Antibody Picoband®

About this product

Boster Bio Anti-MEF2A Antibody Picoband® catalog # PB9953. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9953

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Myocyte-specific enhancer factor 2A is a protein that in humans is encoded by the MEF2A gene. It is mapped to 15q26. The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) . Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

Myocyte-specific enhancer factor 2A; Serum response factor-like protein 1; MEF2A; MEF2

Gene Name

MEF2A

Gene ID

4205

UniProt

Q02078

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2A, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined. .

Applications

IF, IHC, ICC, WB

Field of Research

Calcium Signaling, Calmodulin Pathway, Cardiogenesis, Cardiovascular, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Hypertrophy, Mesenchymal Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Signaling Pathway, Stem Cells, Transcription, Transcription Factors, Transcription Factors/Regulators

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT] (4) TAR-3', found in numerous muscle- specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter. .

References & Citations

1. Bhagavatula, M. R. K., Fan, C., Shen, G.-Q., Cassano, J., Plow, E. F., Topol, E. J., Wang, Q. Transcription factor MEF2A mutations in patients with coronary artery disease. Hum. Molec. Genet. 13: 3181-3188, 2004. 2. Flavell, S. W., Cowan, C. W., Kim, T.-K., Greer, P. L., Lin, Y., Paradis, S., Griffith, E. C., Hu, L. S., Chen, C., Greenberg, M. E. Activity-dependent regulation of MEF2 transcription factors suppresses excitatory synapse number. Science 311: 1008-1012, 2006. 3. Gonzalez, P., Garcia-Castro, M., Reguero, J. R., Batalla, A., Ordonez, A. G., Palop, R. L., Lozano, I., Montes, M., Alvarez, V., Coto, E. The pro279leu variant in the transcription factor MEF2A is associated with myocardial infarction. (Letter) J. Med. Genet. 43: 167-169, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

54811 MW

Observed Molecular Weight

56 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Myocyte-specific enhancer factor 2A

Subcellular Location

Nucleus .

Protein Name

Myocyte-specific enhancer factor 2A

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.