Anti-Elafin/Skalp/PI3 Antibody Picoband®

About this product

Boster Bio Anti-Elafin/Skalp/PI3 Antibody Picoband® catalog # PB9957. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9957

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.

Synonyms

Elafin; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14; PI3; WAP3, WFDC14

Gene Name

PI3

Gene ID

5266

UniProt

P19957

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117) .

Clonality

Polyclonal

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Biology, Cell Cycle, Immunology, Innate Immunity, Kinases/Phosphatases, Lipid Signaling, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway, TLR Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

References & Citations

1. Entrez Gene: PI3 peptidase inhibitor 3, skin-derived (SKALP) . 2. Clauss A, Lilja H, Lundwall A (Nov 2002) . A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein. Biochem J 368 (Pt 1) : 233–42. 3. Molhuizen HO, Zeeuwen PL, Olde Weghuis D, Geurts van Kessel A, Schalkwijk J (Feb 1994) . Assignment of the human gene encoding the epidermal serine proteinase inhibitor SKALP (PI3) to chromosome region 20q12→q13. Cytogenet Cell Genet 66 (2) : 129–31.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

12270 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Elafin

Subcellular Location

Secreted.

Protein Name

Elafin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.