Anti-WDR1 Antibody Picoband®

About this product

Boster Bio Anti-WDR1 Antibody Picoband® catalog # PB9962. Tested in Flow Cytometry, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9962

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

WD repeat-containing protein 1 is a protein that in humans is encoded by the WDR1 gene. It is mapped to 4p16.1. This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

WD repeat-containing protein 1; Actin-interacting protein 1; AIP1; NORI-1; WDR1

Gene Name

WDR1

Gene ID

9948

UniProt

O75083

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human WDR1, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.

Applications

WB, IHC, ICC/IF, Flow Cytometry

Field of Research

Actin Assembly, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PubMed:15629458) . Enhances cofilin-mediated actin severing (By similarity) . Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions (PubMed:18494608) . Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance (By similarity) . Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension (By similarity) . Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt (PubMed:25792565) . .

References & Citations

1. Entrez Gene: WDR1 WD repeat domain 1. 2. Adler HJ, Winnicki RS, Gong TW, Lomax MI (May 1999) . A gene upregulated in the acoustically damaged chick basilar papilla encodes a novel WD40 repeat protein. Genomics 56 (1) : 59–69. 3. Fujibuchi T, Abe Y, Takeuchi T, et al. (2005) . AIP1/WDR1 supports mitotic cell rounding. Biochem. Biophys. Res. Commun. 327 (1) : 268–75.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

66 kDa

Observed Molecular Weight

66 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

WD repeat-containing protein 1

Subcellular Location

Cytoplasm, cytoskeleton . Cell projection, podosome . Cell junction .

Protein Name

WD repeat-containing protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.