Anti-ZP1 Antibody Picoband®

About this product

Boster Bio Anti-ZP1 Antibody Picoband® catalog # PB9970. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9970

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Zona pellucida sperm-binding protein 1 (ZP1) is a protein that belopngs to the mammalian zona pellucida. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. Zp1 ensures the structural integrity of the zona pellucida. Mutations in this gene are a cause of oocyte maturation defect and infertility. And this gene is mapped to chromosome 11q12.2.

Synonyms

Zona pellucida sperm-binding protein 1; Zona pellucida glycoprotein 1; Zp-1; Processed zona pellucida sperm-binding protein 1; ZP1

Gene Name

ZP1

Gene ID

22917

UniProt

P60852

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ZP1, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Clonality

Polyclonal

Tissue Specificity

Oocytes.

Applications

WB

Field of Research

Developmental Biology, Germ Cell Markers, Reproduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.

References & Citations

1. Gross, M. B. Personal Communication. Baltimore, Md. 6/24/2014. 2. Huang, Hua-Lin; Lv, Chao; Zhao, Ying-Chun; Li, Wen; He, Xue-Mei; Li, Ping; Sha, Ai-Guo; Tian, Xiao; Papasian, Christopher J.; Deng, Hong-Wen; Lu, Guang-Xiu; Xiao, Hong-Mei (2014) . Mutant ZP1 in Familial Infertility. New England Journal of Medicine 370 (13) : 1220–1226. 3. Rankin, T., Talbot, P., Lee, E., Dean, J. Abnormal zonae pellucidae in mice lacking ZP1 result in early embryonic loss.Development 126: 3847-3855, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

70049 MW

Observed Molecular Weight

70 kDa, 100 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Zona pellucida sperm-binding protein 1

Subcellular Location

Processed zona pellucida sperm-binding protein 1: Secreted, extracellular space, extracellular matrix . The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida. .

Protein Name

Zona pellucida sperm-binding protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.