Anti-CD33 Antibody Picoband®

About this product

Boster Bio Anti-CD33 Antibody catalog # RP1105. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1105

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.

Synonyms

Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; CD33; Cd33; Siglec3

Gene Name

Cd33

Gene ID

12489

UniProt

Q63994

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived mouse CD33 recombinant protein (Position: D18-E240) . Mouse CD33 shares 61.6% amino acid (aa) sequence identity with human CD33.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

IHC, WB

Field of Research

Cell Type Markers, Hematopoietic Progenitors, Immunology, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid (By similarity) . The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. .

References & Citations

1. Adriaansen, H. J., Geurts Van Kessel, A. H. M., Wijdenes-De Bresser, J. H. F. M., Van Drunen-Schoenmaker, E., Van Dongen, J. J. M.Expression of the myeloid differentiation antigen CD33 depends on the presence of human chromosome 19 in human-mouse hybrids. Ann. Hum. Genet. 54: 115-119, 1990. 2. Brinkman-Van der Linden, E. C. M., Angata, T., Reynolds, S. A., Powell, L. D., Hedrick, S. M., Varki, A. CD33/Siglec-3 binding specificity, expression pattern, and consequences of gene deletion in mice. Molec. Cell. Biol. 23: 4199-4206, 2003. 3. Trask, B., Fertitta, A., Christensen, M., Youngblom, J., Bergmann, A., Copeland, A., de Jong, P., Mohrenweiser, H., Olsen, A., Carrano, A., Tynan, K. Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers. Genomics 15: 133-145, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44824 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Myeloid cell surface antigen CD33

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Name

Myeloid cell surface antigen CD33

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.01 mg NaN3.