Anti-Caldesmon/CALD1 Antibody Picoband®

About this product

Boster Bio Anti-Caldesmon/CALD1 Antibody Picoband® catalog # PB9922. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9922

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Caldesmon is a protein that in humans is encoded by the CALD1 gene. It is mapped to 7q33. This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca (2+) -calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca (2+) -dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Synonyms

Caldesmon; CDM; CALD1; CAD, CDM

Gene Name

CALD1

Gene ID

800

UniProt

Q05682

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Caldesmon recombinant protein (Position: M1-E120) . Human Caldesmon shares 87.6% amino acid (aa) sequence identity with rat Caldesmon.

Clonality

Polyclonal

Tissue Specificity

High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Applications

IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Actin Binding Proteins, Actin, etc., Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments) . Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity) . .

References & Citations

1. Entrez Gene: CALD1 caldesmon 1. 2. Humphrey MB, Herrera-Sosa H, Gonzalez G, et al. (1992) . Cloning of cDNAs encoding human caldesmons. Gene 112 (2) : 197–204. 3. Novy RE, Lin JL, Lin JJ (Oct 1991) . Characterization of cDNA clones encoding a human fibroblast caldesmon isoform and analysis of caldesmon expression in normal and transformed cells. J Biol Chem 266 (25) : 16917–24.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

93231 MW

Observed Molecular Weight

80 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Caldesmon

Subcellular Location

Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) . .

Protein Name

Caldesmon

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.