Anti-ALDH1A2 Antibody Picoband®

About this product

Boster Bio Anti-ALDH1A2 Antibody Picoband® catalog # PB9981. Tested in ICC/IF, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9981

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aldehyde dehydrogenase 1 family, member A2, also known as ALDH1A2 or retinaldehyde dehydrogenase 2 (RALDH2), is an enzyme that in humans is encoded by the ALDH1A2 gene. This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene.

Synonyms

Retinal dehydrogenase 2; RALDH 2; RalDH2;1.2.1.36; Aldehyde dehydrogenase family 1 member A2; Retinaldehyde-specific dehydrogenase type 2; RALDH (II) ; ALDH1A2; RALDH2

Gene Name

ALDH1A2

Gene ID

8854

UniProt

O94788

Host

Rabbit

Reactivity

Human, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ALDH1A2 recombinant protein (Position: M1-A110) . Human ALDH1A2 shares 95.5% amino acid (aa) sequence identity with both mouse and rat ALDH1A2.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.

Applications

ICC/IF, WB

Field of Research

Developmental Biology, Nervous System Development, Neuroscience, Organogenesis, Sensory System, Visual System

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently (By similarity) . .

References & Citations

1. Entrez Gene: ALDH1A2 aldehyde dehydrogenase 1 family, member A2. 2. Ono Y, Fukuhara N, Yoshie O (Dec 1998) . TAL1 and LIM-Only Proteins Synergistically Induce Retinaldehyde Dehydrogenase 2 Expression in T-Cell Acute Lymphoblastic Leukemia by Acting as Cofactors for GATA3. Mol Cell Biol 18 (12) : 6939–50.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57 kDa

Observed Molecular Weight

57 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Retinal dehydrogenase 2

Subcellular Location

Cytoplasm.

Protein Name

Retinal dehydrogenase 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.